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Effect of brefeldin on TAT-NPFlu processing. 51Chromium-release assay using as target cells HeLa B*2705, HT29 B*2705, and B-LCL Bar not pretreated or pretreated for 1 h with brefeldin A (37 μM) prior to incubation for 2 h with TAT-NpFlu (0.1 μM) or Flu 383-391 peptide (30 μM). Two Flu 383-391-specific CTL lines (Bar Flu and Cuc Flu) have been employed as effector cells. The experiment reported here is representative of three.
