TABLE 1.

Stoichiometry of N, M, and HE proteins in immunopurified virions^a

Virus	Relative amt of HE	Relative amt of N	Relative amt of M
MHV-A59	0	1	2.5 ± 0
MHV-HE+	0.38 ± 0.02	1	2.5 ± 0.05
MHV-HE0	0.35 ± 0.005	1	2.7 ± 0.2
MHV-HE−	0	1	2.7 ± 0.1
MHV-S	0.37 ± 0.06	1	2.6 ± 0.2

^aMHV-infected cells were subjected to steady-state labeling with [³⁵S]Met. Virions were immunopurified with a MAb against M or S. Virion proteins were separated by SDS-PAGE. The amount of radioactivity present in each band was determined with a STORM PhosphorImager/Fluorimager 860 (Molecular Dynamics) and ImageQuant software. Relative ratios of HE, N, and M were calculated from the number of Met residues present per protein. The data shown are based on three independent experiments. Data are means ± standard deviations.