FIG. 5.

KSHV activates multiple MAPK pathways during primary infection. Proteins prepared from HUVEC that were mock infected or infected with KSHV at an MOI of 2 for 0.25, 0.5, 1, 2, 4, 8, or 16 h were resolved in a sodium dodecyl sulfate-polyacrylamide gel and transferred onto a nitrocellulose membrane. Phosphorylated or total ERK1/2, JNK, and p38 proteins were detected with the respective specific antibodies. Proteins from HUVEC treated with TPA for 0.5 h were used as positive controls for phosphorylated ERK1/2. Antibodies to α-tubulin were used to normalize sample loading. The experiments were independently carried out two times. The results presented are from one representative experiment.