FIG. 2.

Live SNV replicates in HUVEC, whereas UV-killed SNV does not. HUVEC were treated for 1 hour with SNV at an MOI of 1.0 (squares) or an identical amount of SNV that had previously been exposed to UV irradiation at 254-nm for 15 seconds (triangles). Cells were washed and incubated for the indicated amount of time while medium was changed every other day. Total cellular RNA from confluent T25s (A) or from 140 μl of culture supernatant (B) was extracted at the indicated time points and reverse transcribed using an SNV S-segment forward primer (8). Viral RNA was quantitated by TaqMan using an S-segment primer and probe set and standards of known copy number. PCR was performed in triplicate for duplicate experiments. Results are reported as the mean ± standard error of the mean.