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. 2005 Dec;79(24):15007–15015. doi: 10.1128/JVI.79.24.15007-15015.2005

FIG. 5.

FIG. 5.

SNV exposed to UV irradiation induces ISG transcription despite increased disruption of vRNA. SNV was exposed to UV irradiation for the indicated amount of time and either immediately assayed for S-segment vRNA by TaqMan using a 66-nucleotide target amplimer (7, 8) (open circles and right y axis) or used to treat HUVEC. After treatment for 1 hour, HUVEC were washed and incubated for 6 hours, and total cellular RNA was extracted and used for real-time RT-PCR using p56- and MxA-specific primers (left y axis). Gene changes were normalized to GAPDH and calculated using the 2−ΔΔCt method (see Materials and Methods). PCR was performed in triplicate for duplicate experiments. Results are reported as the mean ± standard error of the mean. *, P < 0.05 compared to mRNA abundance in cells exposed to nonirradiated SNV (0 seconds).