TABLE 1.
Level of replication of the HMPV/AMPV chimeras in the upper and lower respiratory tracts of hamsters
Virusa | Day of harvest | Nasal turbinate
|
Lung
|
||||
---|---|---|---|---|---|---|---|
% with detectable virus | Mean titer (log10 PFU/g of tissue ± ME)b | Reduction of mean titerc (log10 PFU/g of tissue) | % with detectable virus | Mean titer (log10 PFU/g of tissue ± ME) | Reduction of mean titer (log10 PFU/g of tissue) | ||
rHMPV | 3 | 100 | 5.4 ± 0.2 | 100 | 3.7 ± 0.3 | ||
rHMPV-NA | 3 | 83 | 3.1 ± 0.3d | 2.3 | 66 | 1.7 ± 0.0d | 2.0 |
rHMPV-PA | 3 | 100 | 3.6 ± 0.3 | 1.8 | 33 | 1.8 ± 0.1 | 1.9 |
AMPV | 3 | 100 | 5.3 ± 0.1 | 0.1 | 83 | 4.3 ± 0.4 | |
rHMPV | 5 | 100 | 6.5 ± 0.1 | 17 | 1.7 ± 0.0 | ||
rHMPV-NA | 5 | 100 | 5.8 ± 0.2 | 0.7 | 50 | 1.7 ± 0.0 | |
rHMPV-PA | 5 | 100 | 5.1 ± 0.3 | 1.4 | 50 | 2.1 ± 0.2 | |
AMPV | 5 | 83 | 3.4 ± 0.3 | 3.1 | 67 | 1.9 ± 0.1 |
Hamsters in groups of 12 were infected intranasally with 105.7 PFU of the indicated virus on day 0.
Six animals from each group were sacrificed on days 3 and 5. Nasal turbinates and lungs were harvested, and virus titers were determined by plaque assay. ME, mean error.
Reduction of mean log10 titer compared to that of rHMPV.
The value of 50 PFU per g of tissue (the lower limit of virus detection) was assigned to animals lacking detectable virus in order to calculate the mean titer.