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. 2005 Dec;79(24):15114–15122. doi: 10.1128/JVI.79.24.15114-15122.2005

TABLE 4.

Immunogenicity and protective efficiency of rHMPV/AMPV chimeras in AGMs

Immunizing virusa Mean serum neutralizing antibody titer (reciprocal log2 ± SE)b
HMPV replication following challenge on day 28
Preimmunization 28 days postimmunization and statistical groupingc 28 days postchallenge Nasopharyngeal swab
Tracheal lavage specimen
Mean peak titer (log10 PFU/ml ± SE)d Duration of sheddinge (day ± SE) No. of animals shedding virusf Mean peak titer (log10 PFU/ml ± SE) Duration of shedding (day ± SE) No. of animals shedding virus
None (mock) ≤1.5 ≤1.5 6.8 ± 0.3 2.9 ± 0.6 6.0 ± 1.0 2/2 3.6 ± 1.2 5.0 ± 0.0 2/2
rHMPV ≤1.5 6.0 ± 0.7 A 6.0 ± 0.7 0.8 0.5 ± 0.3 2/4 <0.7 0.0 ± 0.0 0/4
rHMPV-NA ≤1.5 5.4 ± 0.4 A 5.8 ± 0.3 <0.7 0.0 ± 0.0 0/4 <0.7 0.0 ± 0.0 0/4
rHMPV-PA ≤1.5 5.0 ± 0.5 A 6.7 ± 0.4 <0.7 0.0 ± 0.0 0/4 <0.7 0.0 ± 0.0 0/4
a

The groups of AGMs are identified by the virus used in the initial immunizing infection as described in Table 3, footnote a. The mock-infected group is an additional group of two animals shown in Fig. 4 that had been mock infected in parallel with the other groups.

b

Sera were collected on days 0 and 28 following the first infection and 28 days following the challenge, and the neutralizing antibody titer against HMPV was determined using an endpoint dilution assay (8).

c

Mean antibody titers were assigned to statistically similar groups by the Tukey-Kramer post hoc test. Values within a column that share a common letter are not significantly different (P < 0.05).

d

On day 28, AGMs from each group were challenged intranasally and intratracheally with 106 PFU of CAN97-83 in 1 ml per site. Nasopharyngeal swabs and tracheal lavage specimens were collected on days 2, 4, 6, and 8 postchallenge, and virus titers were determined by plaque assay. The level of virus replication is expressed as the geometric mean of the peak virus titers (log10 PFU/ml ± standard error) for the animals in each group irrespective of sampling day. The lower limit of detection is 0.7 log10 PFU/ml. A value of 0.7 log10 PFU/ml was assigned to samples without detectable virus.

e

The period of days from the first to the last day on which virus was recovered, including negative days (if any) in between.

f

Number of animals from which virus was detected out of the total number of animals in that group.

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