FIG. 3.

T-cell receptor V_β spectratype analysis of CD8⁺ T lymphocytes. TCR V_β distributions were assessed and compared. (a) Spectratyping was performed using CD8⁺ T lymphocytes from both twins from 12/00. Representative profiles are shown for four V_β families. Of the numerous expanded peaks deviating from Gaussian distribution, a few were common to both twins (e.g., V_β13.2), but most were discordant (e.g., V_β11, V_β15, and V_β22). (b) Spectratyping was performed on CD8⁺ lymphocytes from the twins in 12/00 and 6/01. Spearman correlation coefficients of expanded peaks in intra- and intertwin comparisons are given. Identical analyses of eight unrelated HIV-1-seropositive control subjects (28 interindividual comparisons) revealed a mean correlation coefficient of 0.28 ± 0.19 (data not shown).