FIG. 4.
Peptide-specific T-cell receptor Vβ spectratype analysis. PBMC from 9/01 were cultured in the presence or absence of the Pol peptide HKAIGTVLVGPTPVN (Pol 125-139; protease 69-83), which was the dominantly recognized peptide for both twins (accounting for approximately 50% of the detected HIV-1-specific CTL [data not shown]). Spectratyping then was performed on the CD8+ cells from each twin. Histograms from four representative Vβ families are shown; peptide-stimulated expansions are shaded (peaks whose ratio versus the median of all peaks in the family increased by at least 2 and rose at least twofold after stimulation). The accompanying table indicates the specific peaks within each of the 24 Vβ families that were expanded in response to peptide stimulation. N.D. indicates that data were not available.