FIG. 3.

Binding activities of d(1-8)-SDF-1α (A) and d(1-10)-vMIP-II-(9-68)-SDF-1α (B) to wild-type CXCR4 and mutants. (A) The binding activity of d(1-8)-SDF-1α was reduced by Y45A, F87A, D171A, D187A, E288D, and DNX4. (B) The point mutation of Tyr⁴⁵, Phe⁸⁷, Asp⁹⁷, Tyr¹²¹, Asp¹⁷¹, Asp¹⁸⁷, Tyr²¹⁹, Trp²⁵², Tyr²⁵⁵, Asp²⁶², Glu²⁸⁸, or Phe²⁹² as well as the deletion of the N terminus impaired d(1-10)-vMIP-II-(9-68)-SDF-1α binding. All data are shown as the mean ± standard deviation from at least three independent experiments.