TABLE 1.

Selection pressures in DENV-1 on a gene-by-gene basis^a

Gene	Length (no. of amino acids)	dN	dS	dN/dS	dN/dS Separating genotype I clades (mean)c
Capsid	114	0.012	0.093	0.129	0.061 (0.231)
Membrane	166	0.006	0.189	0.032	0.049 (0.050)
Envelope	495	0.008	0.173	0.046	0.100 (0.056)
Envelopeb	495	0.005	0.086	0.058	0.078 (0.072)
NS1	352	0.007	0.214	0.033	0.029 (0.050)
NS2A	218	0.014	0.235	0.060	0.203 (0.098)
NS2B	130	0.002	0.201	0.010	0.0001 (0.016)
NS3	619	0.005	0.194	0.026	0.019 (0.036)
NS4A	150	0.006	0.200	0.030	0.064 (0.041)
NS4B	249	0.004	0.192	0.021	0.0001 (0.029)
NS5	899	0.006	0.173	0.035	0.042 (0.050)

^aEstimates of d_N, d_S, and d_N/d_S are based on 10 Thai DENV-1 sequences.

^bEstimates are based on a sample of 80 E gene sequences.

^cNumbers in bold indicate cases where the d_N/d_S value for the genotype I clade replacement branches is greater than the mean d_N/d_S value for all branches of this particular gene tree.