TABLE 1.

Primer sequences

No.	ORF	Forward primer (5′→3′)	Reverse primer (5′→3′)	PCR product size (bp)
1	ORF016L	CGAATGCTTGCCGAACGGAT	GCGATATAGTGACAGGCTCC	800
2	ORF018R	ATGGACCGCGCCCTTCATAA	CTACACGGAAGGCACGTCTT	300
3	ORF029R	TATGCCAACGGTCGTGTGCC	TGTCGAGAGCATGGTCCTAA	700
4	ORF033R	TCCAGGAAGTGTATGACCTG	ACTGCATCAGCTCGTGCTGC	1,200
5	ORF037R	GACGCCATCGTCATGGGCAT	AGGCGTGTATATGGCAGGTC	600
6	ORF042R	ATGACTGGTGGCACACCAAA	CCGCAAGCTCAGGTCATTCA	1,000
7	ORF049R	ATGCCTACGGGATCTAGTGC	GACAGCAGGTCCTGCATTAG	500
8	ORF054R	CGCTATGACACGTACATTGC	ACCACATGTCCTGCCATAGC	1,200
9	ORF063R	CGTGTACTGCGGTGGCCATT	GCCATAGCCGTGCGTAACAT	1,200
10	ORF077R	GTCACAGGCCTGTCGCATGT	TTGCGCTTGAGCCCACTCAT	1,200
11	ORF092R	ATGACCACAGGGCACACTGA	CGCGAAATGTCAGCCTGTAC	500
12	ORF097R	ATGCTTGCGCTGGCCATCGT	CCATGACATCATCCGGGTGC	450
13	ORF101R	GCATCCAGACATACTCAGCC	CATGTCCACCAGGTTCACAT	600
14	ORF106R	GCCGAGCACCACTTCCAAGT	CGCACAGGGCTACGCTTTAT	900
15	ORF111R	ATGTCATCGTACCGGTGCCG	GTCCATGTCACGCGCAATGG	900
16	ORF122R	ATAAAGCACCCTCGTGCGGC	CGATGTCCGACAAATGCTCA	300
17	ORF128R	ACTGTCCCAGAATGGAAGAC	CATGCAATGCACAGAGCACC	400
18	ORF135L	ATGAAGTGCACGGCGTTACT	TCCTCGGAGGTGCTGTAGGA	400
19	ORF140R	ATGCGCGTGCTCGAACTATT	TCAACATGTTTTTTGCACAC	600
20	ORF145R	CGTCACAGACGATGTATCCG	ACTCTAGGAGCACGACACGT	900
21	ORF151R	GCCTTTACCAGAGTGCACAC	TGCTCAAGGCCATCGCATGT	800
22	ORF156R	ATTCATTCGAGCCTGCCGGC	GGTGCCCTTACCGTAGAGCT	300
23	ORF161L	ATGGAGTCACAACTTGTCGC	TGTTGCACTGTGGATACGGC	600
24	ORF162R	ACGCAACCGAACAGGCGTGT	CACATGTACCGCAGTGGCGT	1,200
25	ORF171R	GCGTAGACCTACGCGACTGT	GTCCACAGCCATGTGGCACA	1,000
26	ORF179L	TAGGCTGCAGCTGGCCTACA	GGACACGAATGCGACGTCTA	700
27	ORF180R	CGACGTGTCGACCGAAGAGT	TGCGCCTGTGCACAGTAGCA	1,200
28	ORF186R	GACCTTGAAGGCTATGCAGT	GCACTTAGCAGAGCAGAGTC	1,100
29	ORF197L	GCGTCACAGGTACAAGCGCT	GAGAGCCACCTGGTAATGCG	900
30	ORF198R	GCATCCTGCAACACTGTGAG	CGTGTGCTGCACATAGTCGT	800
31	ORF224L	TGCGCATTACGAGCACCTGC	GTGCAGCTGTCGCACCTTGA	1,200
32	ORF226R	GCTCAGGATGTCCATGGCTC	TGGACGACGGCAGCGTCTTA	800
33	ORF234L	AAAGTCCAGCCTGCTGCGCA	CTGGCGCCTTACAGTGTCCA	500
34	ORF237L	ATGTTCCTCGTCAAATGCGG	TACGGCAATGGGTGTTTCTC	400
35	ORF239R	CGCGTCATCTGTCACTCGTG	CAACGCCAGCACAGTGTCCT	1,100
36	ORF256R	GTGTCACCGAAGAGTGGCTG	GCTGGCTTGTGTCGACAACC	800
37	ORF261R	AATTGCATTGCTGCGACCAC	TGAGTTGTACTCACTTTCGC	300
38	ORF268L	ATGTGCTCAGACCAAACGAC	TCAAGCAGGCGATCTGCAAC	800
39	ORF291L	ACGGTACCATCTTGTCTGTC	CACGGGCTATTGAATGGACA	600
40	ORF317L	AGTCGCGTGTTAGCAAGACG	GTTCGCAGCCTTCGTGCTCT	1,100
41	ORF321R	ATGCCGACTACCAAACACAC	CTCAAAGGCGCCGTGATACG	400
42	ORF324R	TCCGAGCTAACACTGTGGCG	GCGCATGAGCCATAATGGCC	600
43	ORF333L	GACCGTGTCGTGTCCATGCT	CGTCAACTCGGCCAATCTCG	600
44	ORF342L	ACTGGAGATTGGCGAAGTGC	TACAGTCGACTGTGCCACTG	1,000
45	ORF349L	CAGGGCAGATACTCTGTGAC	CCATGTCCACGACAGTCTGT	1,300
46	ORF351R	GGACTATATTGCTGAGCTAC	TACAGCTTGATTAGGGCGAC	300
47	ORF353R	GGCATCCTAACATCGCTGGC	CGTTGTCCGTATGCTGCCTG	400
48	ORF373L	CTGCTGACACTGCCCATTGG	GCAGAGCAGCATGTAGAGTC	1,400
49	ORF374R	ATGGGTGCTGCCCAGTCATT	TTATATATGATCGGCCTCGT	1,400
50	MCP (380)	GTACAGCAAGCTGCCCGTTA	ACGGTGACAGTCGATATGGC	500
51	ORF385R	ATGCGTTACGTACCATGCTG	TGCTGTTCGCTTGGCTGAAA	800
52	ORF390R	CACAACGGTGTAGTAACGCG	GACGGTTGCAGGGACCTACA	800
53	ORF394R	ATGCACACAGCGATGAACCT	CACTGTAGTACACACGGTGC	600
54	ORF396R	ATGGCACCGTGTGTACTACA	AGCCATGTGTACATCAGGGC	1,000
55	ORF401R	TGCACACTATGCTGAGTAGC	GGCCATGAGAAACCATGTCA	700
56	ORF407R	GTCTAGTGCCATTGCCATGG	GTAAGCAAGTGCACAGCACC	400
57	ORF412L	AGTTGTCCCTGACGGAGCTG	AAGCCCACATCGGCGTCTGT	700
58	ORF413R	GCCACGTTGATGCACTACAC	TGCGGTGACAAACTGCACGA	400
59	ORF420L	GCTTGCTCTGGGTGATAAGA	GCGCACAATATCATTGGCTG	500
60	ORF423L	GAGACCATCGCCAAGTATGC	CTGCGTATGCGGCCAGCATC	300
61	ORF424R	TTTGCTGCGACCGGAGATGC	CCGTGCTGCCATGGACCTAA	1,300
62	ORF426R	CCGCTTGTTGTCAAGACCAA	ATTGTGGCCTGTGACAACTC	500
63	ORF430L	GCAACTGATGCACGACCGTA	ATCAATGTTCATCGCTGCGC	1,300
64	ORF458L	GTCCGTGACGACCTGTGCAT	TCGACATTGACATGGCGTCG	900
65	ORF463R	ATGCGTCGCGACGGCATGTA	TGTCACCGTTGCCAGTACTG	1,200
66	ORF487L	TGAGCTGCTGTACGCCAACG	ATGCACCTCTACGGTGCCGA	700
67	ORF488R	GCTTGCTATGTGCTACGTGC	TCGCAGACGTGTACCGTGAC	800
68	ORF493R	CGGCTGATCGTCAGCAGTTT	TGTGCCTAGCGCACTGTACA	1,200
69	ORF502R	AGGCAGCCAGCACTGCGTTT	CGGCAGGCTCATGTCAACAC	600
70	ORF506R	CCGCTCAAGACATGTTCGGC	CCAAGGTGTACGCCATGTCG	700
71	ORF515R	AGCCTGTTCCAGCTGCCAGA	TGCGGTTCATTTGCTGTCCC	700
72	ORF522L	GAAGTCTTCCGCTCTGATGC	GGCGAATGTGTGATTGCCC	700
73	ORF534L	GCGCTCAAGGCTGTCTTGCT	AACTGGCGTGCGAACAGCGT	1,000
74	ORF535R	GATGCCTGCACATCTGTCGG	GCATGCTCAAGGGAGTGCAC	500
75	ORF543R	ACGAGACATGTGCCGTGTGC	CAACACAGCAACAGGTACAC	300
76	ORF550R	ACATCGTACGTGGACACCGC	CCCAGGTATGCAAACAGTGG	700
77	ORF554R	AGCGCTGATGAATGACTACC	GCCTCTTCAGCATGAGTGGT	900
78	ORF562R	ATGCATTGTGCCGTGCTCAC	GCATATGTGAGTGCCTCTCC	500
79	ORF569R	ATGATTTCAAGTGCACTGCT	CTAGGCCATAATGCCACGTC	1,000
80	ORF575R	ATGGCCCAGAAGCAGGATAA	CTAGGCAAATGCGGCAATAA	1,000
81	ORF586L	CACTGCAACAGAAGCTACCG	CGCTGCCACAACCGCAGTAT	700
82	ORF589L	TGCGCGGACTGTTCTTCACG	CTGGCAGCATATTCGAGCAT	600
83	ORF591R	GACCTGAGCAAGCTGCCAGT	CATGGTCCAGTCATGCGGCA	400
84	ORF596L	CCGCACGTTTGCGCAGACAA	CCGTCTGATGCTAGCTGTGC	800
85	ORF600L	CGCATGTCTGCAAGCGGTCT	CGCCTGATGTATGCCTCGAT	500
86	ORF606R	ACGCCATTGGACTGCCTGCA	GCGCAGTATAGCATCAAGCG	1,000
87	ORF617L	GTCAAAGTGTGGGCACCATT	CCTTGCATAACGCAGGATGT	400
88	ORF618R	CCGCAGGTTGTGCACTATGT	CGTCGACGGCATGAACGCAT	1,000
89	ORF628L	TATCCAAGGACACTGTGGCG	CACGGCAACAAGCAGTGGCT	500
90	ORF632L	GCGCATCACCGGTACTTGAC	GCACCAGCATACGGTGTTCC	600
91	ORF635L	GTCAGTTGCGCATGCACACC	GCAATAAGCTCTGCCTTGGC	500
92	ORF641L	ATGTCTGCATGGCGTGCAGT	CGTCCGTCTATCGGTGTAAC	1,100