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. 2005 Dec 20;3(2):e17. doi: 10.1371/journal.pmed.0030017

Figure 1. Nitric Oxide Mediates IL-1-Induced β-Cell Death as Determined by the MTT Assay.

Figure 1

RINm5F cells (A and B) or rat islets (C and D) were treated with IL-1 (10 units/ml) and NMMA (2 mM) for either 24 or 48 h, as indicated. The cells were isolated and viability determined using the MTT assay (A and C). The culture supernatants were isolated and nitrite production was determined using the Griess assay (B and D).

(E and F) The effects of exogenously produced nitric oxide supplied by the donor compound DEANO (500 μM) and the apoptosis inducer camptothecin (25 μM) on RINm5F cell viability (E) and the apoptosis inducer staurosporine on rat islet cell viability (F) were determined by MTT assay.

Cell viability data is expressed as percent death. Results for cell viability and nitrite production are the average ± standard error of the mean (SEM) of three independent experiments. *p < 0.05, significantly different from untreated controls. **p < 0.05, significantly different from IL-1-treated condition.