Figure 2. Effects of IL-1 on β-Cell Viability as Determined by Neutral Red Uptake.

RINm5F cells were treated for 24 h or 48 h with IL-1 (10 units/ml), NMMA (2 mM) (A), or camptothecin (25 μM) (B), and the cells were isolated and percentage of death determined using the neutral red uptake assay. Results are average ± SEM of three independent experiments. *p < 0.05, significantly different from untreated controls. **p < 0.05, significantly different from IL-1-treated condition.