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. 2005 Dec 20;3(2):e17. doi: 10.1371/journal.pmed.0030017

Figure 5. IL-1 Stimulates Islet Cell DNA Damage in a Nitric Oxide-Dependent Manner.

Figure 5

Treatment of rat islet cells for 24 h with IL-1 resulted in DNA damage as determined by TUNEL staining, and ∼95% of this damage occurred in insulin-containing cells (red fluorescence) (B). Nitric oxide appears to mediate IL-1-induced DNA damage, as it was attenuated by the NOS inhibitor NMMA (2 mM) (D). The apoptosis inducer staurosporine (1 μM) also stimulated DNA damage in both insulin containing and non-insulin containing cells (C). Less than 10% of control, untreated islet cells were TUNEL-positive (A). Results are representative of three independent experiments.