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. 2005 Dec 20;3(2):e17. doi: 10.1371/journal.pmed.0030017

Figure 6. IL-1 Fails to Activate Caspase-3 in RINm5F Cells or Rat Islets.

Figure 6

(A) IL-1 (10 units/ml), alone or in combination with NMMA (2 mM) and the nitric oxide donor DEANO (500 μM), failed to activate caspase-3 in RINm5F cells following a 24- or 48-h exposure. As a positive control, the apoptosis inducer camptothecin (25 μM) stimulated a 9-fold increase in RINm5F cell caspase-3 activity.

(B) Similar to RINm5F cells, in rat islets IL-1 (10 units/ml) alone or in combination with NMMA (2 mM) failed to activate caspase-3 following a 24- or 48-h incubation. The apoptosis inducer staurosporine (1 μM) stimulated a 5-fold increase in rat islet caspase-3 activity. Results are the average ± SEM of three independent experiments. *p < 0.05, significantly different from untreated control.