Figure 3.

Competitive inhibition of MtuLigA with respect to NAD⁺ by the bis xylofuranosylated diamine with three carbon spacer (Compound 1) and with 1, 3-phenylene carbamoyl spacer (Compound 4). Data were fitted using standard linear regression. The double-reciprocal plots clearly indicate competitive binding between NAD⁺ and compounds 1 and 4. (A) Activity of MtuLigA measured in the presence of rising concentrations of compound 1 (0–250 µM) and NAD⁺ (0–50 µM). (B) Activity of MtuLigA measured in the presence of rising concentrations of compound 4 (0–450 µM) and NAD⁺ (0–50 µM).