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. 2005 Dec 15;33(22):7090–7101. doi: 10.1093/nar/gki1006

Figure 7.

Figure 7

Bactericidal activity of compound 4. Effect on growth as reflected in changes of the optical density at 600 nm of (A) S.typhimurium LT2 and (B) its corresponding DNA ligase minus (null) derivative TT15151[Lig−/T4 Lig+] on their respective exposures to compound 4 at 10–50 µg/ml representing 0.5–2.5 times the MIC value. The arrow indicates the point at which the compound was added. (C) Comparative viability between S.typhimurium LT2 (top row) and its corresponding DNA ligase minus (null) derivative TT15151[Lig−/T4 Lig+](bottom row) rescued with a plasmid containing the gene for T4 Ligase as shown by surviving CFU, 5 h after addition of compound 4 to the growth medium. The cells were plated at dilution ratios of 10−4 on nutrient agar and the indicated MIC values correspond to that of S.typhimurium LT2 against the inhibitor. The control (marked ‘C’) and the amount of added inhibitor as multiples of MIC are also indicated.