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. 2005 Dec 16;33(22):e192. doi: 10.1093/nar/gni191

Figure 4.

Figure 4

Detection of a homozygous deletion by the oligo array CGH platform. DNA from the cell lines MDA-MB-468 (A) and SUM159 (C) was hybridized with normal male reference DNA on a human oligo array. Log2ratios were calculated without moving average and are displayed in black as a function of their position on chromosome 13. The smoothed values of the log2ratios are displayed in red and the position of the RB1 oligo is indicated by the green circle in A and the arrow in C. Note the lack of the deletion in SUM159. Validation of the HD by FISH analysis in cell lines: MDA-MB-468 (B) and SUM159 (D). The green signal from the RB1 probe clearly shows the presence of RB1 in SUM159 (D), but is absent from MDA-MB-468 (B). Chromosome 13 paint (red) shows the presence of three normal copies of chromosome 13 and two marker chromosomes with chromosome 13 material.