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. 2005 Dec 16;33(22):e192. doi: 10.1093/nar/gni191

Figure 5.

Figure 5

Detection of a heterozygous deletion by the oligo array CGH platform. (A) DNA from the cell line SKBR7 was hybridized with normal male reference DNA on a human oligo array. Log2ratios were calculated without moving average and are displayed in black as a function of their position on chromosome 12. The smoothed values of the log2ratios are displayed in red. (B) Validation of the heterozygous deletion on 12q24 by FISH analysis of cell line SKBR7. Chromosome 12 paint (dark blue) shows two copies of seemingly normal chromosome 12. FISH using 3 BACs, RP11-340F14 (red), RP11-44F24 (green) and RP11-7M8 (aqua blue) confirmed the interstitial deletion on one copy of chromosome 12 (arrow). Overlapping FISH signals from different BACs show up in white.