Skip to main content
. 2005 Nov 22;392(Pt 2):313–324. doi: 10.1042/BJ20050636

Figure 6. G0S2 protein localizes to the ER.

Figure 6

HEK-293 cells were co-transfected with GFP–G0S2 fusion construct and ER localization vector pDsRed2-ER. (A) Left-hand panel: confocal image of GFP fluorescence. Middle panel: confocal image of DsRed fluorescence of the same cells as in the left-hand panel. Right-hand panel: overlay of left-hand and middle panels. (B) 3T3-L1 fibroblasts were transfected with fusion constructs of G0S2 to GFP and loaded with lipids by incubation with Tween 80 (0.1%). Lipid droplets were visualized with Oil Red O. (C) 3T3-L1 fibroblasts were transfected with fusion constructs of G0S2 to DsRed and loaded with lipids by incubation with Tween 80 (0.1%). Lipid droplets were visualized with BODIPY® 493/503.