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(A) 3T3-L1 fibroblasts were plated out at low density and grown to full confluence. G0S2 expression was determined by RT-PCR. (B) 3T3-L1 fibroblasts at low confluence were serum-starved (0.2% foetal calf serum) for 33 h. After that, foetal calf serum was re-added to the cells at 10%, and cells were taken at regular intervals for RNA preparation. Expression of the genes indicated was determined by RT-PCR.
