Skip to main content
. 2005 Nov 22;392(Pt 2):335–344. doi: 10.1042/BJ20050465

Figure 6. siRNA-mediated inhibition of Bcl-2, Mcl-1 and c-IAP2 expression and apoptosis.

Figure 6

IEC-6 cells were transfected with either control or the respective siRNA as described in the Experimental section. (A) Expression of Bcl-2, Mcl-1 and c-IAP2. Western blot analysis of control and siRNA transfected cell lysates was performed using Bcl-2, Mcl-1 and c-IAP2 specific antibodies. Membranes were stripped and probed for actin as control for sample loading. Representative blots from three observations are shown. (B) DNA fragmentation. Cells were grown in control medium and transfected with either control siRNA or siRNA specific for Bcl-2, Mcl-1 and c-IAP2. After 48 h transfection the cells were serum-starved, and treated with TNF-α/CHX for 3 h and then subjected to DNA fragmentation analysis. Values are means±S.E.M. *P<0.05 compared with control siRNA transfected cells treated with TNF-α/CHX. OD, absorbance.