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. 2005 Nov 22;392(Pt 2):335–344. doi: 10.1042/BJ20050465

Figure 9. Caspase-3 activation and PARP cleavage.

Figure 9

Cells transfected with vector and DN-STAT3 were grown as described in the Experimental section and treated with TNF-α/CHX or left untreated. Protein (40 μg) from each sample was separated by SDS-gel electrophoresis, transferred to membrane and probed with caspase-3 antibody (A) and a cleaved PARP (Asp-214) rat-specific antibody (B). Membranes were stripped and probed with β-actin antibody as an internal control for equal loading. Representative blots from three experiments are shown.