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. 2005 Nov 22;392(Pt 2):345–352. doi: 10.1042/BJ20051194

Figure 2. Acute induction of IRS1 levels by insulin and feeding in mice.

Figure 2

(A) Mice were fasted overnight and injected intraperitoneally with insulin (150 m-units/g) or saline solution. Skeletal muscle extracts (30–40 μg) were analysed for IRS1 expression by Western blotting, and blots were quantified by densitometry. (B) Mice were fasted for 16 h, and maintained in the fasted state (Fasted) or re-fed (Fed) for 1 h. Liver extracts were analysed for IRS1 expression by Western blotting (upper panel) and quantified (lower panel). Values (means±S.E.M., n=3) are expressed, after correction for β-actin expression, relative to basal expression (saline for muscle and fasted state for liver). *P<0.05 (20 min compared with 40 min, and fasted compared with fed); ***P<0.001 (experimental compared with no insulin).