For details, see the Experimental section. Fatty acids are described as the ‘number of C atoms:number of double bonds (position of first double bond from the methyl end)’, thus C18:2(n−6) is linoleic acid. Values are means±S.E.M. from 11 (ANT-overexpressers, black bars; wild-type, dark grey bars) or six independent preparations (DC701 ANT-underexpressers, white bars; ras59 ANT-underexpressers, light-grey bars). *P<0.05 compared with wild type.