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. 2005 Nov 22;392(Pt 2):399–406. doi: 10.1042/BJ20050580

Figure 2. Effects of GSH and cysteine on redox state of cyt c.

Figure 2

(A) After incubation of oxidized cyt c with 1 mM DTT, GSH or cysteine for 1 h at 37 °C, spectral changes between 370 and 600 nm were measured. The dotted line shows the spectrum of oxidized cyt c in the absence of a reductant; the solid line shows superimposed spectra of oxidized cyt c treated with GSH or cysteine. (B) Absorbance at 550 nm of oxidized cyt c was continuously recorded in the presence of 1 mM GSH or cysteine for 1 h. The spectra corresponded to oxidized cyt c (9 μM) that was incubated with GSH (top trace), cysteine (middle trace) or no reductant (bottom trace).