Skip to main content
. 2005 Nov 22;392(Pt 2):399–406. doi: 10.1042/BJ20050580

Figure 6. Effects of singlet oxygen on redox state of cyt c.

Figure 6

After incubation of either oxidized (A) or reduced (B) cyt c (8 μM) with 0, 1, 3 or 5 mM MNPE for 1 h at 37 °C, spectral changes between 370 and 800 nm were measured. Informative spectral ranges are shown. (A) Spectral traces shown from bottom to top (↑) correspond to oxidized cyt c that was treated with 0, 1, 3 or 5 mM MNPE. (B) Spectral traces shown from top to bottom (↓) correspond to reduced cyt c that was treated with 0, 1, 3 or 5 mM MNPE. (C) Absorbance changes at 550 nm of oxidized or reduced cyt c (8 μM) in the presence of 1 mM MNPE were continuously recorded for 60 min. (D) Spectral changes between 600 and 720 nm are shown. Spectral traces shown from top to bottom (↓) correspond to oxidized cyt c (50 μM) that was incubated with 0 (top), 1 (middle) or 5 (bottom) mM MNPE for 1 h at 37 °C.