Table 1. Steady-state kinetic constants of EGFR tyrosine kinase with peptide substrates derived from ErbB2.
Results represent means±S.E.M.
| Km (μM) | kcat (min−1) | kcat/Km (min−1·mM−1) | ||||||
|---|---|---|---|---|---|---|---|---|
| ErbB2 tyrosine position | Sequence | EGF… | − | + | − | + | − | + |
| 1023 | GDLVDAEEYLVPQQGFF | 215±28 | 228±22 | 2.5±0.1 | 11.7±0.2 | 11.7±1.7 | 52.2±5.0 | |
| 1127 | PLPSETDGYVAPLTCSP | 516±51 | 450±44 | 1.1±0.1 | 4.6±0.1 | 2.1±0.5 | 10.2±1.8 | |
| 1139 | LTCSPQPEYVNQPDVRP | 220±20 | 149±12 | 4.3±0.1 | 18.8±0.2 | 19.5±3.8 | 126±13 | |
| 1196 | GGAVENPEYLTPQGGAA | 280±31 | 168±13 | 2.1±0.1 | 8.4±0.1 | 7.6±1.0 | 49.8±5.1 | |
| 1221/1222 | SPAFDNLYYWDQDPPER | 140±14 | 104±11 | 4.4±0.1 | 16.5±0.1 | 31.8±3.7 | 159±18 | |
| 1248 | TPTVAENPEYLGLDVPV | 197±15 | 122±12 | 1.6±0.1 | 6.5±0.1 | 8.1±1.2 | 53.3±6.7 | |