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. 2005 Dec 6;392(Pt 3):527–536. doi: 10.1042/BJ20050545

Figure 6. Dose-dependent inhibition of proMMP-2 activation by two novel MMPIs in a cell culture system as shown by a representative gelatin zymogram and densitometry analyses using AlphaImager™ 2200.

Figure 6

The conditioned media of HT1080 cells treated with or without different doses of the novel MMPIs plus 20 μg/ml ConA were examined by gelatin zymography. The molecular masses of latent, intermediate and fully activated MMP-2 were demonstrated as 72 kDa pro-MMP-2, 64 kDa int-MMP-2, and 62 kDa act-MMP-2 respectively. Lane 1, HT1080 control; lane 2, HT1080 with ConA; lanes 3–5, HT1080 with ConA and 10, 100 or 200 nM YHJ-132 respectively; lanes 6–8, HT1080 with ConA and 10, 100 or 200 nM YHJ-294-2 respectively.