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. 2005 Dec 6;392(Pt 3):537–544. doi: 10.1042/BJ20050806

Figure 2. ATP transport in mitochondria.

Figure 2

Mitochondria (2 mg of protein/ml) from wild-type (○) or sal1Δ (●) yeast were incubated with 4 mM ATP for 0, 5 or 10 min at 30 °C, and the levels of ATP (A) and ATP+ADP (B) were determined enzymatically. The free calcium concentration of the assay was 6.42 μM. Results are the means±S.E.M. for a representative experiment performed in triplicate. (C) Percentage of nucleotide uptake by the sal1Δ strain after incubation with 4 mM ATP for 5 or 10 min at 30 °C. The uptake of the wild-type strain was considered to be 100%. Results are means±S.E.M. for three independent experiments. The difference between wild-type and sal1Δ strains was significant (P<0.05; Student's t test).