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. 2005 Dec 6;392(Pt 3):537–544. doi: 10.1042/BJ20050806

Figure 4. Sal1p levels in glucose-grown yeast and effects of SAL1 and AAC deletions on viability.

Figure 4

(A) Western-blot analysis of mitochondria (10 μg) extracted from W303, sal1Δ, aac2Δ and aac1,2,3Δ strains grown in YPD medium. (B) Genotypes of the meiotic progeny of diploid yeast heterozygous for SAL1 and AAC1, AAC2 and AAC3 gene disruptions. Three independent sporulation experiments were performed, with a total of 95 tetrads dissected. The results of one representative experiment are shown. Sporulation of heterozygous strains for AGC1 (the aspartate–glutamate carrier) and AAC1,2,3 gene disruptions gave rise to all possible combinations (results not shown), showing that the lethality of the simultaneous disruption in SAL1 and AAC2 is specific.