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Yeast in stationary phase for 2 days were exposed to 2% glucose (□), water (◇) or 2% glucose plus 10 mM EGTA (△) for 2 h and were then transferred to YPD medium. The percentage of cells with buds was determined at various times after transfer to rich medium. Results are means±S.E.M. for three fields, with more than 100 cells/field. The experiment was repeated three times with similar results.
