Table 1. Effects of Mg2+ and Pi on ATP transport.
Influx (a) and efflux (b) of 3H-labelled ATP were assayed for 5 and 10 min in the presence or absence of 5 mM MgCl2 for influx studies, and in the presence or absence of 10 mM Pi, for efflux studies. Results (uptake or loss) are means±S.E.M. for one or two experiments performed in triplicate.
(a) | |||
---|---|---|---|
Mitochondrial ATP (nmol/mg of protein) | |||
MgCl2 concentration (mM) | Incubation time (min)… | 5 | 10 |
0 | 0.73±0.11 | 1.22±0.09 | |
5 | 1.26±0.06 | 1.96±0.09 | |
(b) | |||
Mitochondrial ATP (nmol/mg of protein) | |||
Pi concentration (mM) | Incubation time (min)… | 5 | 10 |
0 | −0.26±0.04 | −0.41±0.08 | |
10 | −0.91±0.09 | −1.04±0.04 |