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. 2005 Dec 6;392(Pt 3):641–648. doi: 10.1042/BJ20051173

Figure 6. AV25 does not inhibit ILK activity.

Figure 6

(A) ILK activity was measured with isolated LC20 as substrate under the indicated conditions, as described in the Experimental section (n=3). Where present, AV25 was at a concentration of 100 μM and staurosporine (STP) at 2 μM; microcystin (MC) was at 1 μM. Values are expressed relative to the control (100%=0.1 nmol of Pi/min per ml) in the absence of kinase inhibitors. Asterisks indicate significant difference from control (P<0.05). (B) Reaction mixtures with LC20 as substrate were analysed by SDS/PAGE and autoradiography. A representative autoradiogram is shown. (C) ILK activity with MLCP (MYPT1) as substrate was assayed by Western blotting with anti-[phospho-Thr-697]-MYPT1 under the indicated conditions. Where present, AV25 was at a concentration of 50 μM.