Table 3. Steady-state metabolite concentrations in erythrocytes evaluated from the mathematical model.
The concentrations of glucose, ATP, ADP, pyruvate, lactate, Mg2+, Pi and G2,3DP were kept constant at 5, 3, 0.3, 0.06, 1.2, 0.3, 1 and 4.5 mM respectively. In model 2, different ADP value was used for the patient (0.6 mM instead of 0.3 mM, while the sum of ADP and ATP was the same). Reference values give the minimal/maximal values of the normal range. While the average haemoglobin content of blood is approx. 9–11 g/dl for the control with a haematocrit of 0.3, all calculations were done at 300 g/l haemoglobin content. The same haemoglobin value was used for the patient. F6P, fructose-6-phosphate; G6P, glucose-6-phosphate; PEP, phosphoenolpyruvate; 2PG and 3PG, 2- and 3-phosphoglycerate.
| Concentration (μM) | |||||
|---|---|---|---|---|---|
| Metabolite | Reference values | Model for control | Measured for patient | Model 1 for patient | Model 2 for patient |
| G6P | 20–110 | 91.1 | − | 72.3 | 72.3 |
| F6P | 6–16 | 28.2 | − | 19.9 | 20.1 |
| FBP | 2–30 | 5.23 | 70±10 | 171 | 79.4 |
| DHAP | 7.6–35 | 19.0 | 720±90 | 266 | 222 |
| GAP | 4.8–20 | 1.94 | − | 6.09 | 2.65 |
| DHAP/GAP | − | 9.79 | − | 43.7 | 83.8 |
| G1,3DP | 0.4 | 1.03 | − | 2.74 | 0.91 |
| 3PG | 55–69 | 336 | − | 887 | 643 |
| 2PG | 5.5–12 | 51.6 | − | 121 | 87.2 |
| PEP | 12–18 | 72.8 | − | 95.9 | 53.3 |