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. 2026 Apr 29;17:1837813. doi: 10.3389/fimmu.2026.1837813

Figure 5.

Panel A shows an experimental timeline and a schematic of a pig undergoing CPR and secukinumab injection, followed by inflammation detection and echo analysis. Panel B presents a fluoroscopy image highlighting a catheter position with an arrow. Panel C displays a bar graph comparing plasma IL-17A levels at baseline and after CA/CPR, showing a significant increase. Panel D contains four bar graphs illustrating relative mRNA amounts of IL1B, IL6, CCL2, and CXCL2, demonstrating significant reductions in the secukinumab group. Panel E features immunofluorescence MPO staining images for isotype and secukinumab treatments, with an adjacent bar graph quantifying reduced positive area in the secukinumab group. Panel F presents a line graph of mean arterial pressure (MAP) over time, showing higher MAP levels for secukinumab-treated subjects. Panel G contains echo images comparing ventricular function between isotype and secukinumab groups. Panel H shows bar graphs for ejection fraction (EF) and fractional shortening (FS), both significantly higher in the secukinumab group.

Early inhibition of IL-17A suppresses myocardial inflammation and improves cardiac function in a porcine CA/CPR model. (A) Schematic representation of the experiment design in pigs. (B) Fluoroscopy image confirmation of successful occupation of temporary pacemaker lead into right ventricular chamber. White arrows indicated temporary pacemaker lead. (C) Plasma levels of IL-17A in baseline and 3 h post-resuscitation in porcine model of CA/CPR. (n = 4). Data were analyzed by Student t test. **P < 0.01. (D) Relative mRNA expression of inflammatory genes in indicated groups after 3 h post-CA/CPR (n = 6). Data were analyzed by Student t test. *P < 0.05; **P < 0.01. (E) Left panel: Representative immunofluorescence images of MPO staining in indicated groups after 3 h post-CA/CPR (n = 4, scale bar = 50 μm). Right panel: Quantification of immunofluorescence staining presented in the left panel (n = 6). Data were analyzed by Student t test. **P < 0.01. (F) MAP in indicated groups. Data were analyzed by 2-way ANOVA followed by the Bonferroni post hoc test. *P < 0.05; **P < 0.01. (G) Representative M-mode echocardiograms of pigs in indicated groups after 3 h post-CA/CPR. (H) Quantification of ejection fraction (EF) and fractional shortening (FS) in indicated groups (n = 6). Data were analyzed by Student t test. **P < 0.01. CA indicates cardiac arrest; CPR, cardiopulmonary resuscitation; IL-17, interleukin-17; MAP, mean arterial pressure; MPO, myeloperoxidase.