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. 2005 Dec 19;102(52):19219–19224. doi: 10.1073/pnas.0504468103

Fig. 3.

Fig. 3.

Expression analysis of System A amino acid transporter genes (Slc38a1, Slc38a2, and Slc38a4) by Northern blotting in Igf2 P0+/- (A) and Igf2 null+/- (B) placentas compared to their wild-types (WT) at two gestational ages (E16 and E19, n = 7 for all WT and mutant groups). Graphs of mean expression levels are shown with the wild-type levels normalized to 1 together with representative Northern blots of total RNA obtained from wild-type (WT) and mutant placentas for Slc38a4 (A), at E16 and E19 and Slc38a2 (B), at E19, compared to Gapd loading. Bars indicate SE. ***, P < 0.001; **, P < 0.01 significantly different from the value in the wild-type. Note increase in signal in the Igf2 P0+/- when compared to WT for the 4-kb transcript only at E16 but not E19.