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. 2005 Dec 19;102(52):18773–18784. doi: 10.1073/pnas.0509487102

Fig. 2.

Fig. 2.

Functional analysis of cLD-Ire1. (A) (Upper) Topography of Ire1 and cLD-Ire1. The Ire1 cLD construct contains an ER-LD starting with amino acid 114 and ending in amino acid 449. (Lower) Immunoblot of HA-tagged Ire1 and cLD-Ire1. (B) Northern blot analysis of HAC1 mRNA in control and DTT-treated cells expressing wild-type Ire1 or cLD-Ire1. Unspliced HAC1u and spliced HAC1i mRNAs are indicated; the lower bands are splicing intermediates. Immunoblot against Hac1-HA in control or DTT-treated cells expressing wild-type (wt) Ire1 or cLD-Ire1. A short exposure (Upper) and a long exposure (Lower) are shown. (C) LacZ activity assay in control cells (gray bars) and DTT-treated cells (black bars) expressing wild-type Ire1 or cLD-Ire1.