Fig. 3.

Analysis of Interfaces 1 and 2. (A) Surface representation the unit cell of cLD monomers in space group P6₅22. There are 24 cLD monomers per unit cell, arranged in two strands that twist around the 6₅ axis. Dashed lines represent the position of interfaces 1 and 2 within the strand between monomers. (B) Ribbon diagram of cLD dimers connected through Interface 1 (Left) or Interface 2 (Right). Dashed lines represent the interfaces between the twofold symmetrical dimers as seen in the asymmetric unit. The red residues shown in stick representation have been mutated. (C) Enlarged view of residues that were mutated (T226W and F247A in Interface 1 and W426A in Interface 2). (D) LacZ activity assay in control cells (gray bars) and DTT-treated cells (black bars) expressing wild-type Ire1 and Ire1 with the indicated mutations. Lower shows an immunoblot of Ire1-HA and its mutant forms.