Table 2. Size determination of cLD in solution.

	Protein mass, kDa
Protein, 10 μM	Predicted	AUC equilibrium sediment	AUC velocity sedimentation	DLS	Gel filtration
cLD	39	43	34	28	34
cLD [T226W, F247A]	39	33	ND	30	44
cLD [W426A]	38	36	ND	30	35

Gel filtration was performed on a Superdex 200 10/30 gel filtration column. AUC, analytical ultracentrifugation; DLS, dynamic light scattering; ND, not detected.