FIG. 2.

TFII-I stable knockdown leads to cell cycle block and decreased cyclin D1. (A) Whole-cell extracts were prepared from control and TFII-I stable knockdown NIH 3T3 cells and normalized by protein concentration. The lysates were analyzed by Western blotting. (B) Control and TFII-I knockdown cells were grown, cells were trypsinized, and their cell cycle profiles were determined by propidium iodide staining followed by FACS analysis using ModFit software.