TABLE 3.
Single mutantsa
| Allele | Original mutant | Anisomycin | Killer | % −1 PRF |
|---|---|---|---|---|
| WT | 1+ | K+ | 8.8 ± 0.6 | |
| S2T | A7 | 3+ | K+ | ND |
| G15C | A44 | 1+ | K− | 14.4 ± 0.7 |
| F16I | A7 | 2+ | K+ | ND |
| L17S | A29 | 2+ | K++ | ND |
| P18S | A20 | 3+ | K++ | ND |
| Y49F | A2 | Hypersensitive | K++ | ND |
| T54A | A16,A23 | 2+ | K++ | ND |
| L99F | A16,A23 | 1+ | K++ | ND |
| A107T | A2 | 2+ | K+ | ND |
| W122G | A36 | 1+ | K++ | ND |
| G141R (Lethal) | A20 | NA | NA | NA |
| H198T | A11 | 1+ | K− | 15.5 ± 0.4 |
| G225S | A4 | 2+ | K++ | ND |
| W255C | A3, prev. study | 4+ | K− | 14.2 ± 0.3 |
| H256Q | A6 | 2+ | K+ | ND |
| P257T | Previous study | 3+ | K− | 13.9 ± 0.4 |
| M261I | A10 | 2+ | K− | 11.3 ± 0.7 |
| I282T | Many alleles | 2+ | K− | 13.2 ± 0.3 |
| Y283C | A4 | 1+ | K++ | ND |
| V312I | A8 | 2+ | K+ | ND |
| E316V | A15 | 1+ | K− | 15.2 ± 0.8 |
| L338S | A36 | 1+ | K++ | ND |
| Y343N | A13 | 1+ | K− | 11.9 ± 0.8 |
| T344S | A4 | Growth enhanced | K++ | ND |
| S347F | A5 | 2+ | K+ | ND |
| K357E | A8 | 2+ | K+ | ND |
| F365Y | A7 | 1+ | K++ | ND |
| K367M | A37 | 2+ | K+ | ND |
| R369S | A12 | 2+ | K++ | ND |
| K384T | A5 | 2+ | K++ | ND |
| K385T | A7 | 3+ | K+ | ND |
| L387F | Many alleles | 2+ | K− | 11.3 ± 0.8 |
a
Tabulation of the anisomycin resistance or sensitivity, killer maintenance, and programmed −1 ribosomal frameshifting profiles of all rpl3 alleles harboring single amino acid changes. Phenotypic scoring and nomenclature are as described for Table 1. NA, not applicable.