FIG. 8.

CHD6 interacts with Nrf2 in mammalian cells and is required for expression of NQO1. (A) Lysates were prepared from Q293 cells expressing HA-tagged Nrf2, Nrf2CTΔ16, Nrf2M1, Nrf2M2, or Nrf2M4 in the presence or absence of a V5-tagged CHD6 protein (amino acids 124 to 324), as indicated. Following IP with immobilized HA antibodies, immune complexes were blotted and probed with V5 or HA antibodies, as specified. A portion of each sample (10%) was removed prior to IP (input) and was blotted and probed with either anti-HA or anti-V5 antibodies. (B to D) HeLa cells were transfected with nonspecific siRNA (Ctrl) or siRNA specific for CHD6 or lamin A/C, as indicated. Subsequently, cells were treated for 24 h with either 0.1% (vol/vol) DMSO (white bars) or 50 μM t-BHQ (black bars). Total RNA was isolated and analyzed by TaqMan with a primer-probe set specific for NQO1 mRNA (B), CHD6 mRNA (C), or Nrf2 mRNA (D). The error bars indicate standard deviations.