TABLE 1.

T-cell populations in wild-type and Prep1^i/i mice^a

Organ	Genotypeb	No. of cells (106)	No. of CD4+ cells (106)	% CD4+	No. of CD8+ cells (106)	% CD8+
Thymus (6-10w.)c (n = 10)	+/+	183.2 (45)	12.3 (2.9)	6.3 (0.8)	2.9 (0.9)	1.6 (0.4)
	i/i	150.4 (35)	5.1 (1.3)	3.2 (0.9)	1.1 (0.4)	0.7 (0.3)
P valued		NS	<0.0005	<0.0002	<0.002	<0.001
Thymus (12-16w.)c (n = 8)	+/+	102.1 (28)	5.5 (1.4)	5.6 (1.1)	1.3 (0.34)	1.4 (0.2)
	i/i	72.3 (26.6)	2.2 (0.6)	2.9 (0.5)	0.45 (0.25)	0.6 (0.13)
P value		<0.1	<0.001	<0.001	<0.001	<0.0002
Spleen (n = 12)	+/+	106.4 (21.7)	27.0 (5.3)	24.3 (4.2)	14.5 (3.4)	12.8 (3.3)
	i/i	120.0 (43.5)	15.1 (3.1)	13.8 (3.3)	7.9 (2.1)	7.1 (2.0)
P value		NS	<0.001	<0.0001	<0.001	<0.00005
Lymph nodes (n = 10)	+/+	23.5 (6.1)	10.1 (4.7)	45.7 (2.0)	7.1 (3.3)	31.5 (1.9)
	i/i	13.6 (5.5)	4.3 (1.7)	36.7 (3.5)	3.3 (0.4)	24.0 (2.7)
P value		<0.01	<0.0001	<0.005	<0.00002	<0.001
Blood (n = 8)	+/+	6.5 (1.1)	2.4 (0.9)	36.2 (4.1)	1.3 (0.3)	20.8 (2.1)
	i/i	6.1 (1.7)	1.4 (0.8)	22.3 (5.0)	0.9 (0.2)	13.4 (2.2)
P value		NS	NS	<0.0005	<0.05	<0.0001

^aThe data shown are mean values. Standard errors of the means are shown in parentheses. The number of cells per organ (thymus, spleen, and lymph nodes) or per milliliter of blood is shown.

^b+/+, wild-type mice; i/i, hypomorphic Prep1^i/i mice.

^cThymi were from 6- to 10-week-old (6-10w.) mice or 12- to 16-week-old (12-16w.) mice.

^dThe P values compare the values for that organ or tissue for the wild-type and Prep1^i/i mice. NS, not statistically significant.