FIG. 2.
Insulin-proliferative and metabolic actions in L6Δ43 cells. (A) L6, L6hIR, and L6Δ43 myoblasts (clones C1, C2, and C8) were stimulated with 100 nM insulin for 10 min as indicated and then solubilized as described in Materials and Methods. Cell lysates (50 μg of protein/sample) were subjected to Western blotting (WB) with phospho-ERK (p-ERK1/2), followed by reblotting with ERK (ERK 1/2) antibodies. Filters were revealed by ECL and autoradiography. The autoradiographs shown are representative of four independent experiments. (B to D) The cells were incubated with 100 nM insulin for 12 h (B) or 10 min (C and D) and assayed for thymidine incorporation into nuclei, 2-deoxy-d-glucose uptake, or glycogen synthase activity as described in Materials and Methods. The bars represent the mean values plus standard deviations of data from three (B), five (C), and four (D) independent experiments, each in triplicate.