FIG. 6.
Requirement of S1P1 receptor for neural development. (A and B) H&E staining of transverse sections from wild-type (A) and S1P1−/− mutant (B) embryos at E12.5. (C and D) TUNEL assay of wild-type (C) and S1P1−/− mutant (D) embryos at E12.5. (E) Percentage of TUNEL-positive cells (n = 6 matched pairs; *, P < 0.01; **, P < 0.05 [paired t test]). (F and G) Immunostaining with anti-phospho-histone H3 of wild-type (F) and S1P1−/− mutant (G) embryos at E12.5. (H) Percentage of phospho-histone H3-positive cells (n = 6 matched pairs; *, P < 0.01 [paired t test]). Scale bars represent 200 μm (A, B, C, and D) and 50 μm (F and G).