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. 2005 Dec;25(24):11073–11088. doi: 10.1128/MCB.25.24.11073-11088.2005

FIG. 2.

FIG. 2.

Elongation of telomeric repeats in tsFT20 at semipermissive temperature. HinfI-digested genomic DNAs isolated from FM3A or tsFT20 cells cultured at 33°C or 38°C for the indicated periods were analyzed for telomeric repeats by Southern hybridization using the telomeric repeat probe (CCCTAA)4. A. tsFT20 exhibited gradual elongation of telomeric repeats during culture at 38°C for 9 weeks. B. Some tsFT20 populations displayed sudden onset of telomeric repeat elongation. C. Some tsFT20 populations displayed gradual telomeric repeat elongation. D. Telomeric repeat elongation does not occur in tsFT20 cells rescued by p180-WT. tsFT20 cells expressing the indicated protein were measured for telomeric repeat length after incubation at 33°C or 38°C for 5 weeks. tsFT20 cells were infected with the control retrovirus vector (vector), the retrovirus encoding the wild-type p180 (p180-WT), or the temperature-sensitive p180 mutant derived from tsFT20 (p180-tsFT20), selected by puromycin treatment, and analyzed.