FIG. 3.

tsFT20 at 38°C showed an extended G-tail before telomeric DNA elongated. A. G-tail signals. DNA was prepared from FM3A or tsFT20 cells that were cultured at 33°C or 38°C for 1 or 9 weeks, treated or untreated with Exo I, and HinfI digested. After electrophoresis, the DNA in the gel was hybridized with the (CCCTAA)₄ probe under nondenaturing conditions. The gel was exposed to a film to obtain an autoradiograph. B. The gel used in panel A was denatured and rehybridized with the (CCCTAA)₄ probe. C. The intensities obtained in panel A were normalized to the intensity of the ∼4-kb signal in panel B, and the relative values are shown such that the value for FM3A or tsFT20 cells cultured at 33°C for 1 week is set as 1.