FIG. 1.

T cell-specific deletion of ILK in mice. (a) ILK^flox/flox mice were bred to transgenic mice expressing Cre recombinase under the direction of the Lck proximal promoter, and the progeny were genotyped by PCR for the inheritance of Cre recombinase and ILK using tail DNA. Representative PCR analyses of deletions in thymocytes from three mice are shown: lane 1, Cre⁻ ILK^wt/flox; lane 2, Cre⁻ ILK^flox/flox; lane 3, Cre⁺/ILK^flox/flox. (b) Western blot analysis of cell lysates from Lck-Cre⁺/ILK^flox/flox mice demonstrated a marked reduction in ILK protein levels in both thymic and splenic T cells, compared to splenic B cells from the same mice, or T cells from thymus and spleen of Lck-Cre⁺/ILK^WT/WT mice (right). Densitometry confirmed a >95% reduction (not shown). Representative data from one of eight independent experiments are shown.